ZFIN Image: Zhang et al., 2024, Figure 4

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Figure Caption

Figure 4

Interspecific chimera formation of hMMES1 in a zebrafish host. (A) Micrograph of zebrafish embryo host (n = 200) showing transplanted hMMES1 (red) and MMES1 (green) at 0, 1, and 3 dpf. Scale bars, 100 μm. (B) Molecular analyses of zebrafish transplanted with MMES1 and hMMES1, wild zebrafish, and medaka embryos at day 1–10 (D1–D10) post-fertilization. The ectoderm markers (nf200 and gfap), mesoderm markers (ntl and myf5), endoderm markers (hnf3b and sox17), and neural crest markers (mitf) were amplified by using primers specific to medaka cDNAs; β-actin of marine medaka or zebrafish was amplified as a reference. All experiments were performed three times, and representative data are shown. The uncropped nucleic acid gels figures are presented in the Supplementary Materials.

Acknowledgments

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