Fig. 5

myd88 overexpression in endothelial cells rescues the fibrotic, scarring and neutrophil phenotypes in cryoinjured myd88?/? ventriclesa, Representative images of immunostaining for EGFP (endothelial cells, magenta) and ?SMA (myofibroblasts, white) with DAPI (DNA marker, blue) counterstaining on sections of cryoinjured Tg(fli1a:myd88,EGFP); myd88+/+ and Tg(fli1a:myd88,EGFP); myd88?/? ventricles at 96 hpci. b, Total number of ?SMA+ cells and of intraventricular ?SMA+ cells in Tg(fli1a:myd88,EGFP); myd88+/+ and Tg(fli1a:myd88,EGFP); myd88?/? injured tissues at 96 hpci. The dots in the graphs represent individual ventricles; data are shown as the mean ± s.d.; n = 6 Tg(fli1a:myd88,EGFP); myd88+/+ and n = 5 Tg(fli1a:myd88,EGFP); myd88?/?. Statistical tests: Student?s t-test. c, Representative images of AFOG staining on sections of cryoinjured Tg(fli1a:myd88,EGFP); myd88+/+ and Tg(fli1a:myd88,EGFP); myd88?/? ventricles at 30 dpci. d, Pie charts showing the proportion of scar components (collagen, blue; fibrin, red; rest of cells and tissue, light brown) in Tg(fli1a:myd88,EGFP); myd88+/+ and Tg(fli1a:myd88,EGFP); myd88?/? scars; n = 6 Tg(fli1a:myd88,EGFP); myd88+/+ and n = 6 Tg(fli1a:myd88,EGFP); myd88?/?. e, Graph showing the percentage of fibrin/scar area at 30 dpci. The dots in the graph represent individual ventricles; data are shown as the mean ± s.d.; n = 6 Tg(fli1a:myd88,EGFP); myd88+/+ and n = 6 Tg(fli1a:myd88,EGFP); myd88?/?. Statistical test: Mann?Whitney U-test. f, Graph showing the representation of groups (y axis) of different scar area sizes (different colors) at 30 dpci for cryoinjured Tg(fli1a:myd88,EGFP); myd88+/+ and Tg(fli1a:myd88,EGFP); myd88?/? ventricles. g, Representative images of immunostaining for EGFP (endothelial cells, magenta) and Mpx (neutrophils, white) with DAPI (DNA marker, blue) counterstaining on sections of cryoinjured Tg(fli1a:myd88,EGFP); myd88+/+ and Tg(fli1a:myd88,EGFP); myd88?/? ventricles at 96 hpci. h, Mpx+ cell numbers in Tg(fli1a:myd88,EGFP); myd88+/+ and Tg(fli1a:myd88,EGFP); myd88?/? injured tissues and border zone areas (100 ?m) at 96 hpci. The dots in the graph represent individual ventricles; data are shown as the mean ± s.d.; n = 6 Tg(fli1a:myd88,EGFP); myd88+/+ and n = 6 Tg(fli1a:myd88,EGFP); myd88?/?. Statistical test: Student?s t-test. The yellow dashed lines delineate the injured area; the black dashed lines delineate the scar area; the yellow arrowheads point to ?SMA+ (a) and Mpx+ (g) cells. Scale bars, 100 ?m (a,g), 200 ?m (c).