Fig. 7

Hair cell number following regeneration is slightly reduced in IFT gene mutants. Representative images of neuromasts with hair cells labeled using the otoferlin antibody in (A) ift88 and (B) dync2h1 wild-type siblings (left) and mutants (right), pre (top) and post (bottom) regeneration. Scale bars: 10 µm. Quantification of hair cells/neuromast for (C) ift88 and (D) dync2h1 wild-type siblings and mutants during the regeneration experiment. EM groups were fish that were kept in EM the first 24 h of the experiment whereas Gent groups were fish that were put in 200 µM gentamicin for the first 24 h of the experiment. 0 h groups were fixed immediately following those first 24 h, whereas 48 h groups were fixed following a 48 h recovery period in EM. A three-way ANOVA was carried out for both experiments which found all variables (time, drug, genotype) to be significant sources of variation as well as all two-way interactions between the three variables (P<0.001). The three-way interaction between variables was found to be significant for the dync2h1 experiment (P=0.003), but not the ift88 experiment (P=0.796). ****=P<0.0001 and ***=P<0.001 by Šídák's multiple comparisons test comparing the two groups at the edges of the lines under the asterisks. The 48-h gentamicin data shown in C and D is also shown in panels E for ift88 and F for dync2h1 following subtraction of the average 0-h gentamicin data for each group. There was a significant difference between ift88 wild-type siblings and mutants ****=P<0.0001, but not for dync2h1 (P=0.1639) by unpaired t-test following this normalization. n=10 for all groups.