Fig. 1

ift88 mutants show decreased hair cell number and hair cells undergoing apoptosis. (A) Representative images of neuromasts from ift88 wild-type siblings (top) and ift88 mutants (bottom) at 6 dpf. Nuclei are labeled in blue with DAPI, hair cells in green with the Otoferlin antibody and cells undergoing apoptosis in magenta with the cleaved caspase-3 antibody. Scale bar: 10 µm. (B) Quantification of hair cells/neuromasts in ift88 wild-type siblings and mutants at 4, 5 and 6 dpf. Genotype and age were significant sources of variation by two-way ANOVA (P<0.0001), but the interaction between the two variables was not (P=0.2377). ****=P<0.001 by Šídák's multiple comparisons test comparing the wild-type siblings and mutants at the different ages. n=10 for each group. (C) Quantification of the total number of cleaved caspase-3 positive hair cells in the nine neuromasts of the posterior lateral line in ift88 wild-type siblings and mutants at 4, 5 and 6 dpf. Genotype was a significant source of variation by two-way ANOVA (P=0.001) whereas age (P=0.1089) and the interaction between the two variables (P=0.0189) were not. n=10 for each group.