FIGURE

Fig. 3

ID

ZDB-FIG-240828-40

Publication

Caetano da Silva et al., 2024 - Functional analysis of ESRP1/2 gene variants and CTNND1 isoforms in orofacial cleft pathogenesis

Other Figures

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Fig. 3

Functional testing of human ESRP1 and ESRP2 gene variants.A Representative images of the ANC from Alcian-blue stained larvae at 4 dpf after injection with esrp2 MO and 200 pg of: esrp1 mRNA, esrp2 R353Q mRNA. ANC was scored as a rescued ANC or cleft ANC (B) ESRP1 and (C) ESRP2 gene variant rescue assay results for embryos injected with esrp2 MO and 200 pg of esrp1 variant mRNA. Results presented as percentage of rescue vs. cleft as different numbers of embryos survived and were analyzed, indicated as n above each bar. D ESRP1 and (E) ESRP2 gene variant rescue assay by detecting alternative splicing of Arhgef11 in murine Py2T wildtype and Esrp1−/−; Esrp2−/− double knockout cells.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	esrp1^fb401/fb401 + MO1-esrp2
Knockdown Reagent:	MO1-esrp2
Observed In:	ceratohyal cartilage Meckel's cartilage
Stage:	Day 4

Phenotype Detail

Acknowledgments

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