FIGURE

Fig. 6

ID

ZDB-FIG-240828-34

Publication

Maekawa et al., 2024 - The Oct4-related PouV gene, pou5f3, mediates isthmus development in zebrafish by directly and dynamically regulating pax2a

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Transcriptional regulatory activity of the pax2a upstream DNA analyzed by in vivo EGFP reporter assay. (A) Schematic view of the pax2a 10-kb upstream DNA with six NCSs ( Fig. 5 ). Below are shown the DNA sub-fragments whose transcriptional regulatory activities were examined by in vivo EGFP reporter assay, in which sub-fragments were co-injected into embryos with hsp-egfp DNA. BP, basic promoter; PDE, pax2a distal enhancer; PPE, pax2a proximal enhancer. (B) EGFP fluorescence in the embryo regions was evaluated and scored at 24 hpf. n, number of embryos examined; HB-MN, hindbrain motor neuron; MHB, midbrain-hindbrain boundary region; MH-HB, broad expression in the midbrain and hindbrain; NSE, non-specific expression; SC-MN, spinal cord motor neuron. ???, ?+?, ?++?, and ?+++? indicate expression rates of 0%, 1?10%, 11?30%, and 31?70%, respectively (see Table 2 , Table 3 ). Grey boxes indicate relatively high specific expression rates.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cells Dev