FIGURE

Fig. 2

ID

ZDB-FIG-240828-30

Publication

Maekawa et al., 2024 - The Oct4-related PouV gene, pou5f3, mediates isthmus development in zebrafish by directly and dynamically regulating pax2a

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Fig. 2

Time course of the expression of MHB-related genes in embryos in which en-pou5f3 was induced at late gastrulation. Embryos obtained by crosses of en-pou5f3+/− and wild-type fish were subjected to heat shock at 90 % epiboly (9 hpf) and examined for the expression of MHB-forming genes (A, pax2a; B, fgf8a; C, wnt1; D, en2a; and E, gbx2) by WISH from 1 to 4 hours post-heat shock (hphs). As references, the expression of six3b in the forebrain and/or egr2b in the hindbrain was assessed. Dorsal views are shown with anterior to the top. Solid and open arrowheads mark normal and reduced expression at the MHB, respectively. Arrows mark the ectopic gbx2 expression in the forebrain. For all marker genes and genotypes, at least 7 embryos were examined, all of which showed the indicated patterns. Additional data are shown in Fig. S1 . anb, anterior neural boundary; fb, forebrain; op, otic placode; r1–5, rhombomeres 1–5. Scale bars, 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

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