FIGURE

Fig. 1

ID

ZDB-FIG-240827-1

Publication

Krueger et al., 2024 - Heritable CRISPR Mutagenesis of Essential Maternal Effect Genes as a Simple Tool for Sustained Population Suppression of Invasive Species in a Zebrafish Model

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Fig. 1

A heritable CRISPR mutagenesis strategy leading to sustained sex-specific disruption of reproduction as a simple and efficient method for population suppression in fish. Schematic of the HMEL strategy. Left: A CRISPR knockin cassette with ubiquitous expression of Cas9 and a gRNA targeting an essential maternal effect gene - here npm2b - is integrated in the genome. Right: Fish inheriting the knockin, called HMEL fish, express CRISPR nuclease causing npm2b mutant maternal embryo lethal phenotype (red) in females, while fertile HMEL males (yellow) can pass on the construct. HMEL, heritable maternal effect embryo lethality; npm2b, nucleoplasmin 2b; WT, wildtype.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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