PUBLICATION
Heritable CRISPR Mutagenesis of Essential Maternal Effect Genes as a Simple Tool for Sustained Population Suppression of Invasive Species in a Zebrafish Model
- Authors
- Krueger, C.J., Dai, Z., Zhu, C., Zhang, B.
- ID
- ZDB-PUB-240322-1
- Date
- 2024
- Source
- Zebrafish 21(4): 279-286 (Journal)
- Registered Authors
- Zhang, Bo
- Keywords
- invasive species, maternal effect gene, population suppression, sterile male release technique, zebrafish
- MeSH Terms
-
- Male
- Maternal Inheritance
- Female
- CRISPR-Cas Systems*
- Zebrafish*/genetics
- Mutagenesis*
- Introduced Species*
- Animals
- Zebrafish Proteins/genetics
- PubMed
- 38512221 Full text @ Zebrafish
Citation
Krueger, C.J., Dai, Z., Zhu, C., Zhang, B. (2024) Heritable CRISPR Mutagenesis of Essential Maternal Effect Genes as a Simple Tool for Sustained Population Suppression of Invasive Species in a Zebrafish Model. Zebrafish. 21(4):279-286.
Abstract
Invasive species control is important for ecological and agricultural management. Genetic methods can provide species specificity for population control. We developed heritable maternal effect embryo lethality (HMEL), a novel strategy allowing negative population pressure from HMEL individuals to be transmitted within a population across generations. We demonstrate the HMEL technique in zebrafish through genome-integrated CRISPR/Cas targeted mutagenic disruption of nucleoplasmin 2b (npm2b), a female-specific essential maternal effect gene, causing heritable sex-limited disruption of reproduction. HMEL-induced high-efficiency mutation of npm2b in females suppresses population, while males transmit the HMEL allele across generations. HMEL could be easily modified to target other genes causing sex-specific sterility, or generalized to control invasive fish or other vertebrate species for environmental conservation or agricultural protection.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping