CM082 suppressed retinal neovascularization induced by hypoxia. (A) Survival rate of zebrafish embryos exposed to CM082 from 0 to 200 μM under normoxia (n = 100 in each group). (B) Survival rate of zebrafish embryos in various media under normoxia or hypoxia (n = 100 in each group). (C–J) The retinal vessels in various media under normoxia and hypoxia conditions were imaged (n = 6 in each group). (K, N) The pattern shows the method for measuring and counting vessel diameters (red line), ratio of the area of IV relative to IRV (IV, white line; IRV, blue line) and branch points (yellow asterisks). The vessel diameter and the IV/IRV ratio were measured using Image J. (L) There was no significant difference on the vessel diameter among all the 8 groups. (M, O) Under normoxia, the number of branch points and the IV/IRV ratio were not significantly different among the 4 groups with various media. However, these two values were significantly higher in the hypoxia groups with EM or DMSO compared to the normoxia groups. Under hypoxia, this discrepancy was mitigated by 0.5 and 1 μM CM082. NRV, nasal radial vessel; DRV, dorsal radial vessel; VRV, ventral radial vessel; IV, intraocular vessel; IRV, intraocular ring vessel. Scale bar = 100 μm *p < 0.05; **p < 0.01; ***p < 0.001. Data represent the mean ± SEM.
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