FIGURE

Fig. 5

ID

ZDB-FIG-240806-5

Publication

Li et al., 2024 - Threonine dehydrogenase regulates neutrophil homeostasis but not H3K4me3 levels in zebrafish

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Fig. 5

tdh knock-out slightly attenuates neutrophil migration induced upon acute injury. (A) Experimental scheme of the tail transection assay used in this study. (B, C) Left: brightfield and fluorescence microscopy images of the wound site of 2 dpf (B) or 3 dpf (C) WT//Tg (lyz:EGFP) and tdh−/−//Tg (lyz:EGFP) transgenic zebrafish embryos at 6 h post transection in the presence of N-phenylthiourea (PTU). Right: quantification analysis of EGFP+ cell numbers, and percentages of the EGFP+ cells in wound site compared to the whole CHT in WT//Tg (lyz:EGFP) and tdh−/−//Tg (lyz:EGFP) transgenic zebrafish embryos. (D) The same experiments were performed using the WT//Tg (mpx:EGFP) and tdh−/−//Tg (mpx:EGFP) transgenic zebrafish embryos in the absence of PTU. Scale bar = 500 μm in (B) to (D). Data are presented as the mean ± SD; two-tailed t-test; ***P < 0.001; **P < 0.01.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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