Fig. 1

The mRNA expression level of tdh is relatively high in neutrophils of zebrafish compared to several acetyl-CoA metabolism related genes. (A) Schematic diagram of some pathways contributing to mitochondrial acetyl-CoA pool in zebrafish. (B) Heatmap showing the mRNA expression levels of enzymes or transporters involved in different metabolic pathways in each distinct cell type. Note that the mRNA expression levels are normalized across samples of each row so that they do not reflect absolute expression levels of these genes. Rela. Exprs, relative expression. scRNA-seq data was download from Zebrafish Blood Viewer Website (https://molpath.shinyapps.io/zebrafishblood/). (C) tSNE plot showing 5 distinct clusters and cells were colored by their cell type annotation in right. (D) tSNE visualization of the mRNA expression levels of some marker genes for cell-type identification. zfpm1and tal1 (HSPCs), lyz and mpx (neutrophils), il17r and themis (T cells), cd37 and igic1s1 (B cells), and zbtb32 and nkl.2 (NK cells) were selected to distinguish each subgroup. (E) tSNE visualization of the expression patterns of tdh (left) and tdh2 (right) in each distinct subgroup. (F) Dot plot showing the expression of key enzymes or transporters involved in glycolysis or mitochondrial acetyl-CoA generation in each distinct cell type. The scRNA-seq data shown in (C) to (F) are from the GEO dataset GSE100911. (G) Heatmap showing the mRNA expression levels of tdh and several related genes in broad types of tissues of WT zebrafish embryo at 24 h post fertilization (hpf). Note that tdh and gcat are ubiquitously expressed but shows relative enrichment in certain tissues, whereas tdh2 and sdsl have almost no expression at this developmental stage. lyz, mpx and cebpb are known markers for neutrophils/myelocytes. The scRNA-seq data exhibited in (G) are from the ZESTA database zf24_scRNA.h5ad dataset (https://db.cngb.org/stomics/zesta/).