Fig. 6

ttnaΔ9/Δ9 zebrafish embryos exhibit ANP upregulation and ANP-dependent changes in IKs channel components and atrial function (A) Quantification of mRNA levels for nppa, kcnq1, and kcne1 in zebrafish embryos at 48 hpf. Each dot represents a biological replicate, and each replicate contains 5–10 embryos. (B) Quantification of mRNA levels for kcne1 in negative control morpholino (Control MO)-treated or morpholino targeting nppa (nppa MO)-treated ttnaΔ9/Δ9 zebrafish embryos at 48 hpf. Each data point represents a biological replicate, and each replicate contains 5–10 embryos. (C) Quantification for atrial contraction of Control MO-treated (n = 47) or nppa MO-treated (n = 38) ttnaΔ9/Δ9 zebrafish embryos at 48 hpf. (D) Quantification for atrial contraction of Control MO-treated (n = 19) or nppa MO-treated (n = 17) ttna+/+ zebrafish embryos at 48 hpf. (E) Quantification of ventricular contraction of Control MO-treated (n = 26) or nppa MO-treated (n = 17) ttnaΔ9/Δ9 zebrafish embryos at 48 hpf. Data are represented as mean ±95% confidence intervals. ns p > 0.05; ∗p < 0.05; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001. See also Figures S9 and S10 .