Fig. 3

A. baylyi T6SS-medatied antagonism towards E. coli induces host inflammation and reduces host survival. (A) Enumeration of recovered E. coli or A. baylyi at 0, 6, or 24 hpi from zebrafish larvae coinfected with a 1:1 mixture (~2000 CFU each) of E. coli and WT A. baylyi (Ec x Ab WT) or E. coli and ΔT6SS A. baylyi (Ec x Ab ΔT6). Circles represent individual larvae. Data were pooled from three independent experiments, each with 3–5 larvae per time point. Significance was assessed using unpaired t-test on Log10 transformed values. (B) Fold change in the expression of cxcl8a, tnfa, il1b, mmp9 and cxcl18b in larvae coinfected with a 1:1 mixture (~2000 CFU each) of either E. coli and WT A. baylyi (Ec x Ab WT) or E. coli and ΔT6SS A. baylyi (Ec x Ab ΔT6SS) relative to mock infected larvae at 6 hpi, 18 hpi, or 42 hpi. Data were pooled from three independent experiments, each with 10–15 larvae per time point. Significance was assessed using unpaired t-test on Log2 transformed values. (C) Survival curves of larvae coinfected with a 1:1 mixture (~2000 CFU each) of either E. coli and WT A. baylyi (Ec x Ab WT) or E. coli and ΔT6SS A. baylyi (Ec x Ab ΔT6). Data were pooled from three independent experiments, each with 10–15 larvae. Significance was assessed by log-rank Mantel-Cox test. For all panels, bars indicate mean ± SEM. *p < 0.05, ****p<0.0001, and ns (not significant). https://doi.org/10.1371/journal.ppat.1012384.g003