PUBLICATION
Use of zebrafish to identify host responses specific to type VI secretion system mediated interbacterial antagonism
- Authors
- Virgo, M., Mostowy, S., Ho, B.T.
- ID
- ZDB-PUB-240720-3
- Date
- 2024
- Source
- PLoS pathogens 20: e1012384e1012384 (Journal)
- Registered Authors
- Mostowy, Serge
- Keywords
- none
- MeSH Terms
-
- Acinetobacter
- Vibrio cholerae*/pathogenicity
- Antibiosis/physiology
- Virulence
- Animals
- Type VI Secretion Systems*/metabolism
- Rhombencephalon/metabolism
- Rhombencephalon/microbiology
- Zebrafish*/microbiology
- Host-Pathogen Interactions
- PubMed
- 39024393 Full text @ PLoS Pathog.
Citation
Virgo, M., Mostowy, S., Ho, B.T. (2024) Use of zebrafish to identify host responses specific to type VI secretion system mediated interbacterial antagonism. PLoS pathogens. 20:e1012384e1012384.
Abstract
Interbacterial competition is known to shape the microbial communities found in the host, however the interplay between this competition and host defense are less clear. Here, we use the zebrafish hindbrain ventricle (HBV) as an in vivo platform to investigate host responses to defined bacterial communities with distinct forms of interbacterial competition. We found that antibacterial activity of the type VI secretion system (T6SS) from both Vibrio cholerae and Acinetobacter baylyi can induce host inflammation and sensitize the host to infection independent of any individual effector. Chemical suppression of inflammation could resolve T6SS-dependent differences in host survival, but the mechanism by which this occurred differed between the two bacterial species. By contrast, colicin-mediated antagonism elicited by an avirulent strain of Shigella sonnei induced a negligible host response despite being a more potent bacterial killer, resulting in no impact on A. baylyi or V. cholerae virulence. Altogether, these results provide insight into how different modes of interbacterial competition in vivo affect the host in distinct ways.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping