FIGURE

Fig. 4

ID

ZDB-FIG-240725-39

Publication

Niu et al., 2024 - FBLN2 is associated with Goldenhar syndrome and is essential for cranial neural crest cell development

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Fig. 4

Design and knockout efficacy evaluation of guide RNAs (gRNAs). (A) Structure of zebrafish fbln2 and gRNA target site design. Untranslated regions are marked by white boxes, and coding regions are marked by orange boxes. Scissors represent the target site of the four gRNAs. (B) Verification of induced mutations carried by four gRNA-microinjected embryos (F0) near the target sites by Sanger sequencing. (C) Knockout efficacy calculation of the gRNAs. The R2 and p values were calculated by the software TIDE to assess the goodness of fit and significance of detection. Alleles with p < 0.001 were considered significant and shown in red, among which the unmodified alleles were shown in a lighter shade. Abbreviation: WT, wild type.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Ann N Y Acad Sci