FIGURE

Fig. 4

ID

ZDB-FIG-240715-89

Publication

Ligunas et al., 2024 - Tissue-specific and endogenous protein labeling with split fluorescent proteins

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Combinatorial expression of tissue-specific mNG21-10 and mNG211-tagged proteins. A. Schematic of crossing strategy. tsp, tissue-specific promoter. poi, protein of interest. B-D. Representative images of embryos obtained from crossing mNG211-tubb4b and ubb:mNG21-10 (B–B′), fez2f:mNG21-10 (C–C′), or myl7:mNG21-10 (D). B′ and C′ show boxed regions in B and C, respectively. E-H. Representative images of embryos obtained by crossing krt8-mNG211 to ubb:mNG21-10 (E–F), fez2f:mNG21-10 (G), or myl7:mNG21-10 (H). E′ shows boxed region in E. Maximum projections of confocal z-stacks. Images were acquired at 24 h post-fertilization (hpf) unless otherwise noted. Image in D has been overexposed to emphasize lack of fluorescence. Autofluorescent speckles (yolk, pigment cells, and debris) are colored blue for display purposes. Scale bars, 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 514, Ligunas, G.D., Paniagua, G., LaBelle, J., Ramos-Martinez, A., Shen, K., Gerlt, E.H., Aguilar, K., Nguyen, N., Materna, S.C., Woo, S., Tissue-specific and endogenous protein labeling with split fluorescent proteins, 109-116, Copyright (2024) with permission from Elsevier. Full text @ Dev. Biol.