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Fig. 4 Combinatorial expression of tissue-specific mNG21-10 and mNG211-tagged proteins. A. Schematic of crossing strategy. tsp, tissue-specific promoter. poi, protein of interest. B-D. Representative images of embryos obtained from crossing mNG211-tubb4b and ubb:mNG21-10 (B?B?), fez2f:mNG21-10 (C?C?), or myl7:mNG21-10 (D). B? and C? show boxed regions in B and C, respectively. E-H. Representative images of embryos obtained by crossing krt8-mNG211 to ubb:mNG21-10 (E?F), fez2f:mNG21-10 (G), or myl7:mNG21-10 (H). E? shows boxed region in E. Maximum projections of confocal z-stacks. Images were acquired at 24 h post-fertilization (hpf) unless otherwise noted. Image in D has been overexposed to emphasize lack of fluorescence. Autofluorescent speckles (yolk, pigment cells, and debris) are colored blue for display purposes. Scale bars, 50 ?m.
Reprinted from Developmental Biology, 514, Ligunas, G.D., Paniagua, G., LaBelle, J., Ramos-Martinez, A., Shen, K., Gerlt, E.H., Aguilar, K., Nguyen, N., Materna, S.C., Woo, S., Tissue-specific and endogenous protein labeling with split fluorescent proteins, 109-116, Copyright (2024) with permission from Elsevier. Full text @ Dev. Biol.