?-Glucan training alleviates LPS-induced pyroptosis in zebrafish liver cells (A) Lateral fluorescence images of PBS- and ?-glucan-trained Tg (fabp10a: dsRed) larvae after LPS challenge. The livers of zebrafish larvae are indicated with white dashes. Scale bar, 150 ?m. (B) Statistical quantification of liver fluorescence (n = 10) as indicated in (A). (C) TEM analysis of the ultrastructure of liver cells in PBS- and ?-glucan-trained larvae after LPS challenge. Scale bar, 5 ?m. (D) Images of immunofluorescence in PBS- and ?-glucan-trained larvae after LPS challenge using TUNEL staining, TUNEL (green), and DAPI (blue). The inside liver area is circled with a white dotted line and labeled with ?L,? and the intestine area is labeled with ?I.? Scale bar, 100 ?m. (E) Western blot analysis of Caspy2/GSDMEb in PBS- and ?-glucan-trained larvae after LPS challenge. FL, full-length, NT, N-terminal, ?, non-specific bands. (F) Quantification of relative protein level in (E) through grayscale analysis. Data are replicated with three independent experiments and shown as mean ± SD by a two-way ANOVA with multiple comparisons. ?p < 0.05, ???p < 0.001. See also Figure S2.
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