Fig. 8

Analysis of autophagy pathway after trehalose and SG2 administration. qRT-PCR analysis of hypoxia-inducible factor 1-alpha (hif-1α). qRT-PCR analysis was performed (normalizing to β-actin) in untreated cln8−/− larvae compared with cln8−/− larvae treated with trehalose and SG2 at 5 dpf. Three independent RNA samples (each obtained from about 30–40 larvae) from controls and cln8−/− mutant larvae at 5 dpf 120 hpf were analyzed. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, calculated by Student's t-test. The values are expressed as mean ± standard error of the mean (SEM). (B) Three independent larval homogenates from WT (n = 50), untreated cln8−/− (n = 50) and treated cln8−/−larvae were tested by Western blotting for the expression of LC3, Rab7 and cathepsin D protein. The levels of the protein were normalized to β-actin. * p ≤ 0.05, **p ≤ 0.01, calculated by one-way ANOVA test. The values are expressed as mean ± SEM.