mfsd12a morpholino in ctns−/− embryos reduced cystine levels. A and B: ctns−/− embryos were injected with 100 µM, 200 µM, or 400 µM mfsd12a translation-blocking morpholino’s (TB MO). Cumulative mortality (A) and dysmorphism (B) were quantified. Average n = 75.9 (ctns−/−), 40.5 (+100 µM NC MO), 54.2 (+200 µM NC MO), 50.0 (+100 µM mfsd12a TB MO), 62.2 (+200 µM mfsd12a TB MO), and 81.7 (+400 µM mfsd12a TB MO). Median with 95% CI is shown for mortality rate and means ± SE for dysmorphism. Significance was tested by a Kruskal–Wallis test and one-way ANOVA, respectively. ***P < 0.001; ****P < 0.0001. C: both pericardial edema (top) and/or a curved spine (bottom) were counted. D: cystine levels were assessed at 72 h post-injection of mfsd12a TB MO and compared with the ctns−/− and ctns+/+ zebrafish by Kruskal–Wallis tests. Each dot represents a single measurement (n = 8 for ctns+/+, n = 15 for ctns−/− , n = 7 for ctns−/− + 100 µM NC MO and mfsd12a MO, n = 11 for ctns−/− + 200µM NC MO and mfsd12a MO). *P < 0.05; **P < 0.01; ****P < 0.0001.
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