Fig. 3

mfsd12a morpholino in ctns?/? embryos reduced cystine levels. A and B: ctns?/? embryos were injected with 100 µM, 200 µM, or 400 µM mfsd12a translation-blocking morpholino?s (TB MO). Cumulative mortality (A) and dysmorphism (B) were quantified. Average n = 75.9 (ctns?/?), 40.5 (+100 µM NC MO), 54.2 (+200 µM NC MO), 50.0 (+100 µM mfsd12a TB MO), 62.2 (+200 µM mfsd12a TB MO), and 81.7 (+400 µM mfsd12a TB MO). Median with 95% CI is shown for mortality rate and means ± SE for dysmorphism. Significance was tested by a Kruskal?Wallis test and one-way ANOVA, respectively. ***P < 0.001; ****P < 0.0001. C: both pericardial edema (top) and/or a curved spine (bottom) were counted. D: cystine levels were assessed at 72 h post-injection of mfsd12a TB MO and compared with the ctns?/? and ctns+/+ zebrafish by Kruskal?Wallis tests. Each dot represents a single measurement (n = 8 for ctns+/+, n = 15 for ctns?/? , n = 7 for ctns?/? + 100 µM NC MO and mfsd12a MO, n = 11 for ctns?/? + 200µM NC MO and mfsd12a MO). *P < 0.05; **P < 0.01; ****P < 0.0001.