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The zAvatar-test can be used to test alternative therapy options. a–c zAvatar#41CCU was treated in vivo with FOLFOX (chemotherapy regimen) and FOLFIRI (alternative regimen) and compared with untreated controls. d–f zAvatar#64CCU was treated in vivo with CAPOX (chemotherapy regimen) and CAPIRI (alternative regimen) and compared with untreated controls. For all examples, apoptosis (b, e) and tumor size (c, f) were analyzed at 3dpi. Tumor cells are labeled in white (Deep Red), activated Caspase 3 in magenta, DAPI in blue and human-mitochondria marker in green. Data is expressed as AVG ± SEM. Each dot represents one zAvatar and the total number (n) of zAvatars analyzed is indicated in the images. Dashed white line is delimitating the tumor of each zAvatar. Scale bars represent 50 µm. Dashed line in the graphs is marking the 1.34 threshold defined by the ROC curve (b, e). Data were analyzed using unpaired two-sided Mann–Whitney test: (ns) > 0.05, *p = 0.0403, **p = 0.0082. g Examples of zAvatars tested with the chemotherapy taken by the patient, their clinical outcome and the alternative option. Blue depicts sensitivity and red resistance to treatment. Source data are provided as a Source Data file.
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