FIGURE

Fig. 7

ID

ZDB-FIG-240603-42

Publication

Wang et al., 2023 - Artificial Lipid Biomembranes for Full-Length SARS-CoV-2 Receptor

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Fig. 7

Expression of human ACE2-740 (hACE2-740) and its binding to spike protein of SARS-CoV-2. a) Structure of human ACE2 protein (Length: 805 aa). ACE2 is a single TM type I membrane protein, 18?615 aa is the peptidase domain (PD), and 616?805 aa is the collectrin-like domain (CLD) that contains the neck domain, a long linker, TM region, a single TM (TM) helix, and an ?40-residue intracellular segment. The TM region connects the cytosolic side to the extracellular side. b) Structure of ACE2-RBD complex; orange and ochre colors represent RBD protomers. c) Representation of the ELISA used to detect the binding of ACE2 to RBD. d) Western blot of cell-free expression of hACE2-740, and the O.D. values of binding of cell-free expressed hACE2-740 and commercial hACE2-740 to RBD, as determined by ELISA (initial concentration: 0.5 mg mL?1). Data presented as mean � SD, n = 3.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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