Fig. 6

Cell-free membrane protein expression systems using nanodiscs. a) The assembled nanodiscs supported the membrane protein after being expressed in cell-free reactions. b) Effects of serial concentrations of four lipids on cell-free expression. Data presented as mean ± SD, n = 3. c) Effects of serial concentrations of four lipids with scaffold protein MSP1E2 on cell-free expression in a nanodisc environment. Data presented as mean ± SD, n = 3. d) Expression of membrane proteins in a cell-free system with the addition of nanodiscs composed of four lipids and three scaffold proteins. Dark columns represent the fluorescence intensity of total protein, and light columns represent the fluorescence intensity of soluble (supernatant) protein. Data presented as mean ± SD, n = 3.