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Fig. 3

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ZDB-FIG-240603-25

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Hattori et al., 2012 - A fluorescence-detection size-exclusion chromatography-based thermostability assay for membrane protein precrystallization screening

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Fig. 3

Effects of Additives on the Thermostability of zfP2X4 (A) The normalized peak heights from EGFP-fusion ΔP2X4-A, heat-treated at 50°C for 10 min, in the presence of the respective additives. The peak heights were normalized to that from the sample at 4°C without any additive. (B–D) Chemical structures of novel cholesterol derivatives, hexaethyleneglycol hydroxyethyl cholesterol (B) maltoside hydroxyethyl cholesterol (C), and phosphocholine hydroxyethyl cholesterol (D), employed in the FSEC-TS studies are shown. Structures were created using the ChemDraw (Cambridge Scientific Computing). See also Figure S2 and Table S1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Structure (London, England : 1993), 20, Hattori, M., Hibbs, R.E., Gouaux, E., A fluorescence-detection size-exclusion chromatography-based thermostability assay for membrane protein precrystallization screening, 129312991293-9, Copyright (2012) with permission from Elsevier. Full text @ Structure