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Fig. 2
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- ZDB-FIG-240603-24
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- Hattori et al., 2012 - A fluorescence-detection size-exclusion chromatography-based thermostability assay for membrane protein precrystallization screening
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Fig. 2
Thermostability Profiles of zfP2X4 and GluCl (A, C, and E) Representative FSEC profiles from EGFP (A), EGFP-ΔP2X4-A (C), and GluClcryst (E), heated at the respective temperatures, detected by GFP fluorescence (A and C) or by Trp fluorescence (E). (B, D, and F) Representative melting curves of EGFP (B), EGFP-tagged and EGFP-free ΔP2X4-A (D), and GluClcryst and GluCl-EGFP (F). Melting temperatures (Tm) were determined by fitting the curves to a sigmoidal dose-response equation. The fitted curves are shown as a black line. See also Figure S1. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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Reprinted from Structure (London, England : 1993), 20, Hattori, M., Hibbs, R.E., Gouaux, E., A fluorescence-detection size-exclusion chromatography-based thermostability assay for membrane protein precrystallization screening, 129312991293-9, Copyright (2012) with permission from Elsevier. Full text @ Structure