FIGURE

Fig. 2

ID

ZDB-FIG-240601-2

Publication

Xiao et al., 2024 - mTOR mutation disrupts larval zebrafish tail fin regeneration via regulating proliferation of blastema cells and mitochondrial functions

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Fig. 2

Construction of mTOR knock out zebrafish using CRISPR/Cas9 technology. (A) The gRNA targeted sequences in the exon 4 of mTOR gene. (B) Sanger sequencing results after injection of Cas9 mRNA. (C) DNA gel electrophoresis was used to detect PCR products. (D) Schematic diagram showing the screening of mTOR^−/− fish line through Self-fertilization. (E-F) The fin area of un-amputated mTOR-WT and mTOR-KO zebrafish at 3 dpf. (G) Statistical analysis of p-S6/S6 between mTOR-WT and mTOR-KO larval zebrafish fin after amputation. (H) Effect of mTOR knock out on larval zebrafish fin regeneration after amputation. (I-J) Statistical analysis of fin regeneration area and rate after fin amputation between mTOR-WT and mTOR-KO zebrafish larvae. ^nsP > 0.05, ****P < 0.0001

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J Orthop Surg Res