Fig. 2

Fig. 2

Construction of mTOR knock out zebrafish using CRISPR/Cas9 technology. (A) The gRNA targeted sequences in the exon 4 of mTOR gene. (B) Sanger sequencing results after injection of Cas9 mRNA. (C) DNA gel electrophoresis was used to detect PCR products. (D) Schematic diagram showing the screening of mTOR^−/− fish line through Self-fertilization. (E-F) The fin area of un-amputated mTOR-WT and mTOR-KO zebrafish at 3 dpf. (G) Statistical analysis of p-S6/S6 between mTOR-WT and mTOR-KO larval zebrafish fin after amputation. (H) Effect of mTOR knock out on larval zebrafish fin regeneration after amputation. (I-J) Statistical analysis of fin regeneration area and rate after fin amputation between mTOR-WT and mTOR-KO zebrafish larvae. ^nsP > 0.05, ****P < 0.0001