Activation of dopamine D2-like receptors modulates visual size representation in the tectum (A) Images showing part of the tectum of a food-deprived 7 dpf elavl3:H2B-GCaMP6s larva before and after treatment with quinpirole. Scale bars, 50 μm. (B and C) Graphs depicting cumulative percentages of WMR angles of pcp4a+ (B) or pcp4a− (C) PVNs in unfed 7 dpf elavl3:H2B-GCaMP6s larvae before and after 3-h treatment with quinpirole or in control unfed 7 dpf elavl3:H2B-GCaMP6s fish not treated with quinpirole but kept in agarose for the same duration of the drug treatment. n = 5 fish per group. (D) Confocal images showing localization of pcp4a, vglut2, and gad1b mRNA in tectal PVNs in 7 dpf elavl3:gal4, UAS:EGFP larvae. Scale bars, 10 μm. (E) Bar graph showing percentages of glutamatergic (vglut2+) or GABAergic (gad1b+) pcp4a+ PVNs. n = 6 larvae per group. (F and G) Graphs showing cumulative percentages of WMR angles of pcp4a+ and gad1b+ (F) or pcp4a+ and gad1b− (G) PVNs in unfed 7 dpf elavl3:H2B-GCaMP6s larvae before and after 3-h treatment with quinpirole or in control unfed 7 dpf elavl3:H2B-GCaMP6s fish not treated with quinpirole, imaged in two consecutive sessions 3 h apart. Data in these graphs are subsets of the data shown in (B). n = 4 fish per group. ∗∗∗p < 0.001; n.s., not significant; two-way ANOVA. Data are shown as mean ± standard error of the mean.
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