Mutating pcp4a alters decision to approach or avoid small visual stimuli (A) Schematic representation of the strategy for mutating the pcp4a gene using the CRISPR-Cas9 technique. The truncated protein, if produced, would lack the Calmodulin-interacting domain (red box in the wild-type protein sequence). (B) Scheme of the behavioral setup for recording approach/avoidance decisions of zebrafish larvae. (C) Graph depicting average valence indexes for different sizes of visual stimuli of 7 dpf unfed pcp4a+/+ and pcp4amd78/md78 larvae. (D) Graphs showing approach (top) or avoidance (bottom) probability for small visual stimuli in 7 dpf unfed pcp4a+/+ and pcp4amd78/md78 larvae. Approach and avoidance probabilities were calculated as (approaches/[approaches + avoidances + neutral interactions]) or (avoidances/[approaches + avoidances + neutral interactions]), respectively. For approach probability, p = 0.7 (1°), p = 0.9 (3°), and p = 1.0 (5°). For avoidance probability, p = 0.02 (1°), p = 0.2 (3°), and p = 0.04 (5°). (E) Graph depicting average activity indexes for different sizes of visual stimuli of 7 dpf unfed pcp4a+/+ and pcp4amd78/md78 larvae. In (C)–(E), npcp4a+/+ = 22 larvae, npcp4amd78/md78 = 18 larvae. ∗p < 0.05; n.s., not significant; two-tailed t test with Bonferroni-Holm correction. (F) Bar graph showing average spontaneous locomotion of 7 dpf unfed pcp4a+/+ and pcp4amd78/md78 larvae. npcp4a+/+ = 27 larvae, npcp4amd78/md78 = 19 larvae. n.s., not significant; two-tailed t test. Data in (C)–(F) are shown as mean ± standard error of the mean.
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