Fig. 3

PDGFRB somatic mutation induce phenotypic modulation in SMCs. Immunostaining reveals the expression levels of smooth muscle markers (a-SMA and SM22a) and inflammatory markers (VCAM1, ICAM1, MMP1 and MMP9) in HBVSMCs transfected with different viruses (Control: vector; Wild Type: PDGFRB; Y562D: PDGFRB^Y562D) (a). The relative density of immunoblot bands about markers shown in (A) were display (B) (normalized to those in cells transfected with vector viruses). RT-qPCR (C) of SMCs markers (α-SMA and SM22α) and inflammatory markers (VCAM-1, ICAM1, MMP-9 and MMP-1) in HBVSMCs underwent different treatments. Student's t-test and Benjamini–Hochberg correction are employed to assess the statistical significance. Edu assay exhibit the proliferation ability of HBVSMCs under different treatment conditions (D). Statistical analysis of the proportion of Edu-positive cells in the different groups from 5 different fields of each group at × 200 magnification. Tukey's multiple comparisons test is used for statistical differences (E). Scratch assay displays migratory ability of HBVSMCs underwent different treatment (F). Statistical analysis of the rate of wound healing (reduced area at 4H /area at 0H) in the different groups from 9 different fields of each group at × 200 magnification. Tukey's multiple comparisons test is utilized to evaluate the statistical significance. *padj < 0.05, **padj < 0.01, ***padj < 0.001, ****padj < 0.0001 (G). The above experiments are all repeated three times