FIGURE

Fig. 3

ID

ZDB-FIG-240509-86

Publication

Moshal et al., 2019 - LITAF Regulates Cardiac L-type Calcium Channels by Modulating NEDD4-1 Ubiquitin Ligase

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Fig. 3

Control of LTCC current (ICa,L) and Cavα1c (L-type calcium channel alpha-1C subunit) protein levels by LITAF (lipopolysaccharide-induced tumor necrosis factor) in 3-wk-old cardiomyocytes. Cardiomyocytes were transduced with adenovirus encoding GFP (green fluorescence protein) or HA (hemagglutinin)-LITAF for 48 h. A, Left, voltage steps to measure I-V for ICa,L. Right, representative recordings of ICa,L in cardiomyocytes. B, Voltage dependence of ICa,L current density in cardiomyocytes transduced with GFP or LITAF (cells from 5 animals; mean±SEM; Student t test, P<0.05). C, Protein levels of Cavα1c, HA-LITAF, and tubulin ( left). Respective change in Cavα1c abundance, normalized to tubulin (n=5 animals, performed in triplicate; mean±SEM). Student t test, P<0.05 ( right). D, Current-voltage relationships of ICa,L peak currents for baseline conditions from cells expressing scrambled RNA or short hairpin RNA (shRNA) against endogenous LITAF (cells from 5 animals; mean±SEM; Student t test, P<0.05). E, Protein levels of Cavα1c, total LITAF, and tubulin ( left). Respective changes in Cavα1c and LITAF abundance, normalized to tubulin (n=5 animals, performed in triplicate; right).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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