Fig. 2

BAPN induced AAD-related pathological changes in adult zebrafish. A, the survival rate of the zebrafish during the 22-day administration period is presented through statistical analysis and a significant difference of ***<0.001. B, a notable distinction can be observed between the zebrafish group administered with BAPN and the control group on the 22nd day after three months of treatment. It is evident that the treatment of BAPN leads to the occurrence of multiple blood vessel bleedings in the body and abnormal changes in the development of the notochord. C, the differences observed after the administration of BAPN are depicted in the masson, TB, and HE stained sections. The scale bars in the images represent a measurement of 100 μm (100 x). D, based on the pathological changes of zebrafish dorsal aorta, the incidence of AAD after treatment of zebrafish BAPN was statistically analyzed, n = 12; E and F, statistics of relative area and wall thickness of blood vessels in pathological sections; G and H, elastin staining showed fragmentation grades. Grading keys are shown on the left (p < 0.05), Scale bars: 100 μm (100 x). Data are presented as mean ± SEM. *p < 0.05.