FIGURE

Fig. 6

ID

ZDB-FIG-240419-55

Publication

Li et al., 2024 - A natural small molecule aspidosperma-type alkaloid, hecubine, as a new TREM2 activator for alleviating lipopolysaccharide-induced neuroinflammation in vitro and in vivo

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Hecubine suppressed LPS-triggered activation of the NF-κB p65 pathway in BV-2 cells. Cells were pretreated with Hecubine for 1 h and then stimulated with/without LPS. (a–d) The levels of total/phosphorylated IKKα/β (b), IκB α (c), and P65 (d) were measured via Western blot (b, n = 3; c, n = 4; d, n = 3). (e–f) The expression levels of total NF-κB p65 in the cytoplasmic fraction (n = 5) (e) and nuclear fraction (n = 4) (f) were quantified by Western blot. (g) The nuclear translocation of p65 was evaluated by immunofluorescence analysis. Cells were stained with anti-p65 antibody (green) and DAPI (blue). – and + represent the absence and presence of LPS (800 ng/ml), respectively. The data are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. LPS-treated group; ###p < 0.001 vs. control; One-way ANOVA with Dunnett’s multiple comparisons test. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Redox Biol.