FIGURE

Fig. 6

ID

ZDB-FIG-240415-19

Publication

Stergas et al., 2023 - CRK and NCK adaptors may functionally overlap in zebrafish neurodevelopment, as indicated by common binding partners and overlapping expression patterns

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Fig. 6

CRK and NCK family SH2 domains have overlapping neuronal binding partners involved in cell migration. (A) The 29 common SH2-binding partners from Fig. 5 were categorized by molecular function and protein-type. Proteins that were categorized as cytoskeletal components, cell adhesion, adaptor/scaffold molecules, or motility/polarity/chemotaxis proteins were placed in the table. Also provided are total spectral counts from CRK-SH2 and NCK-SH2 MS. (B) CRK and NCK family SH2 domains are induced by H2O2 to interact with CAS, a major component of focal adhesions. Anti-CAS western blot of whole-cell extract (WCE) and the indicated pulldowns using extracts from Neuro2A cells treated with (+) or without (−) H2O2. The anti-pY blot shows effective stimulation by H2O2. *Indicates full-length GST-SH2 fusion (breakdown products are major bands below). Molecular weight markers in kilodaltons are indicated.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ FEBS Lett.