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Fig. 6 CRK and NCK family SH2 domains have overlapping neuronal binding partners involved in cell migration. (A) The 29 common SH2-binding partners from Fig. 5 were categorized by molecular function and protein-type. Proteins that were categorized as cytoskeletal components, cell adhesion, adaptor/scaffold molecules, or motility/polarity/chemotaxis proteins were placed in the table. Also provided are total spectral counts from CRK-SH2 and NCK-SH2 MS. (B) CRK and NCK family SH2 domains are induced by H2O2 to interact with CAS, a major component of focal adhesions. Anti-CAS western blot of whole-cell extract (WCE) and the indicated pulldowns using extracts from Neuro2A cells treated with (+) or without (−) H2O2. The anti-pY blot shows effective stimulation by H2O2. *Indicates full-length GST-SH2 fusion (breakdown products are major bands below). Molecular weight markers in kilodaltons are indicated.