FIGURE

Fig. 7

ID

ZDB-FIG-240415-20

Publication

Stergas et al., 2023 - CRK and NCK adaptors may functionally overlap in zebrafish neurodevelopment, as indicated by common binding partners and overlapping expression patterns

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Fig. 7

CRKL-SH3-1 and NCK1-SH3-3 share overlapping neuronal interacting partners involved in cell migration. (A) Representative coomassie-stained gels used for MS analysis of GST, GST-CRKL-SH3-1, and GST-NCK1-SH3-3 pulldown experiments using extracts from Neuro2A cells. Molecular weight markers in kilodaltons are indicated. (B) Comparison of CRKL-SH3-1 (dark green) and NCK1-SH3-3 (light green) interactors found in at least two replicates reveals 23 overlapping interactors. Binding partners involved in cell migration are shown in the overlap. (C) The 23 common SH3-binding partners were characterized by molecular function and protein-type. Proteins that were categorized as cytoskeletal components, adaptor/scaffold molecules, or motility/polarity/chemotaxis proteins were placed in the table. Also provided are total spectral counts from CRKL and NCK1 MS runs. (D) CRKL-SH3-1 and NCK1-SH3-3 both interact with WASL, an actin-binding protein involved in cell migration. Anti-WASL western blot of whole-cell extract (WCE) and GST, GST-CRKL-SH3-1, and GST-NCK1-SH3-3 pulldowns using Neuro2A lysate. Ponceau-stained membranes indicate lysate before pulldowns (left panel) and pulldowns (right panel). Molecular weight markers in kilodaltons are indicated.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ FEBS Lett.