FIGURE

Fig. 3

ID

ZDB-FIG-240410-3

Publication

Oliveira Silva et al., 2024 - Donepezil-Loaded Nanocarriers for the Treatment of Alzheimer's Disease: Superior Efficacy of Extracellular Vesicles Over Polymeric Nanoparticles

Other Figures

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Fig. 3

Uptake of EVs and NPs-PLA-PEG by bEnd.3 and SK-N-SH cells. (A) Schematic representation of staining of EVs and NPs-PLA-PEG by PKH26 and Cy5, respectively. Created with BioRender.com. (B) Representative of nFCM fluorescence intensity resulting from staining EVs with PKH26 (red) or NPs-PLA-PEG with Cy5 (blue). Black signal resulted from EVs and NPs-PLA-PEG without fluorescence labelling (control). Flow cytometric analysis of EVs-PKH26 or NPs-PLA-PEG-Cy5 uptake by bEnd.3 (C and D) and SK-N-SH (E and F) cells after 24h of incubation. Controls represent cells that were not incubated with any formulation (untreated). The filtrate from the last wash after labeling and the unlabeled nanoformulations (EVs and NPs-PLA-PEG) were used to determine the background. The percentages of PKH26 and Cy5 positive cells are indicated inside the gates.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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