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Fig. 5.

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ZDB-FIG-240408-48

Publication

Chen et al., 2024 - col1a2⁺ fibroblasts/muscle progenitors finetune xanthophore countershading by differentially expressing csf1a/1b in embryonic zebrafish

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Fig. 5.

scRNA-seq reveals the sources of Csf1a and Csf1b in embryonic zebrafish.

(A) Scheme of the sample preparation workflow. Trunks of 27- to 28-hpf embryos were dissected and dissociated into single cells. (B) Clustering of the single-cell transcriptome results in 30 clusters (label with different colors and no. 0 to 29). (C and D) Expression analysis of csf1a in t-SNE plot (C) and violin plot (D). The results showed that csf1a expression is largely restricted to cells in clusters 2 and 3. (E and F) Expression analysis of csf1b in t-SNE plot (E) and violin plot (F). The results showed that csf1b expression is largely restricted to cells in cluster 2. (G) Heatmap of normalized differentially expressed genes: csf1a, csf1b, col1a2, and col5a1 in all 30 clusters. (H) Relative expression levels of csf1a and csf1b in Col1a2⁺ and Col1a2⁻ cells, respectively. Col1a2⁺ and Col1a2⁻ cells (n = 50,000, respectively) were sorted from Tg (col1a2: Gal4; UAS: NTR-mCherry). Data represented four independent experiments. Error bars represent mean ± SD. Unpaired, two-tailed t test was performed to determine significance. *P < 0.05 and **P < 0.01.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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