Figure 2
- ID
- ZDB-FIG-240405-54
- Publication
- Zhou et al., 2024 - Activating SRC/MAPK signaling via 5-HT1A receptor contributes to the effect of vilazodone on improving thrombocytopenia
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VLZ promotes megakaryocyte differentiation and enhances the DNA ploidy of HEL and Meg-01 cells. (a, b) Representative images of HEL and Meg-01 cells treated with different concentrations of VLZ (2.5, 5, and 10 μM) for 5 days. Bars represent 100 μm. The positive control was PMA. (c, d) Giemsa staining of HEL and Meg-01 cells treated with VLZ (2.5, 5, and 10 μM) or PMA for 5 days. Bars represent 25 μm. (e, f) Phalloidin staining of HEL and Meg-01 cells treated with VLZ (2.5, 5, and 10 μM) or PMA for 5 days. DAPI staining nuclei (blue) and Phalloidin staining of F-actin (red). Bars represent 100 μm. (g, h) Flow cytometry analysis of the percentage of CD41+/CD42b+ complexes surface expression on HEL and Meg-01 cells by VLZ (2.5, 5, and 10 μM) or PMA for 5 days. The histogram shows the percentage of CD41+/CD42b+ cells for each group. (i, j) Flow cytometry analysis of the DNA ploidy of HEL and Meg-01 cells treated with VLZ (2.5, 5, and 10 μM) or PMA for 5 days. The histogram shows the percentages of DNA ploidy. (k, l) Flow cytometry analysis of the cell apoptosis of HEL and Meg-01 cells treated with VLZ (2.5, 5, and 10 μM) or PMA for 5 days. Data are shown as the mean ± SD from three independent experiments. *p≤0.05, **p≤0.01, and ***p≤0.001, vs the control group. |