FIGURE

Fig 5

ID

ZDB-FIG-240310-5

Publication

Ruan et al., 2024 - New insights into the all-testis differentiation in zebrafish with compromised endogenous androgen and estrogen synthesis

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Fig 5

Fancl promoted K48-linked ubiquitination of Tp53 in HEK293T cells.

(A) Transfection of Myc-tagged Fancl decreased the levels of Flag-tagged Tp53 in a dose-dependent manner. (B) Quantification of the western blot bands of the target protein, Tp53. (C) The proteasome inhibitor, MG-132, blocked the Fancl-mediated Tp53 destabilization. (D) Quantification of the western blot bands of the target protein, Tp53. (E) Fancl promotes K48-linked ubiquitination, rather than K6-, K11-, K27-, K29-, K33-, K63-linked ubiquitination of Tp53. IP, immunoprecipitation. IB, immunoblotting. TCL, total cell lysate. Different letters in the bar charts represent significant differences.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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