ZFIN Image: Ruan et al., 2024, Fig 5

Image

Figure Caption

Fig 5 Fancl promoted K48-linked ubiquitination of Tp53 in HEK293T cells.

(A) Transfection of Myc-tagged Fancl decreased the levels of Flag-tagged Tp53 in a dose-dependent manner. (B) Quantification of the western blot bands of the target protein, Tp53. (C) The proteasome inhibitor, MG-132, blocked the Fancl-mediated Tp53 destabilization. (D) Quantification of the western blot bands of the target protein, Tp53. (E) Fancl promotes K48-linked ubiquitination, rather than K6-, K11-, K27-, K29-, K33-, K63-linked ubiquitination of Tp53. IP, immunoprecipitation. IB, immunoblotting. TCL, total cell lysate. Different letters in the bar charts represent significant differences.

Acknowledgments

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