Gene expression analysis in regenerating gill filaments using RT-qPCR. (A–E) Relative mRNA expression of fgf8a, bmp2b, bmp6, her6 and jag1b in regenerating gills of zebrafish 10 days after the resection procedure (squares), compared with expression in gills from intact animals (circles). Expression of all genes was observed under control conditions, but relative abundance increased during regeneration for only fgf8a, bmp2b and her6. Data were normalized to the mRNA abundance of the reference gene, ef1a. Data were analyzed using the Mann–Whitney U-test (two-tailed) and means±s.e.m. significantly different from control are indicated by asterisks (U=0, P<0.01; N=8 for each group). n.s., not significant.
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