Pharmacological inhibitors reduced the area of gill filament regrowth during regeneration. The distal tips of gill filaments from zebrafish exposed to (A) water or (B) DMSO as controls, or to (C) SU5402, (D) DMD or (E) LY411575 are shown at 5 dpr. A large region of tissue regrowth was observed in controls (A,B) but was reduced in drug treatment groups. In all images, the dashed line indicates the site of resection. F, filament; L, lamella. Scale bar: 50 µm and applies to all panels. (F) Summary data showing means±s.e.m. area of the filament tip (in µm2) at 5 dpr for control and DMSO groups (circles), and for drug treatment groups (squares). Data were analyzed by one-way ANOVA and Tukey's test (F=56.97, P<0.05; N=10 for each group). Significant differences between groups are indicated by different lowercase letters.
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