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Implantation of EOC ZTX models in zebrafish larvae. a Illustration of digestion, staining, implantation, and visualization of EOC tumour samples from primary tumour and metastases transplanted to zebrafish larvae. b Quantification of changes in tumour size (rel. size) between day zero and day three for 19 samples from the primary tumour in 19 patients. Cut-off for included models in further studies was those that decreased less than 80% in size (red dotted line). n is shown as individual dots in the graph. c Quantification of changes in tumour size (rel. size) between day zero and day three for 8 samples from metastatic lesions in 8 patients. Cut-off for including models in further studies were a decrease of less than 80% in size (red dotted line). n is shown as individual dots in the graphs. The experiment was done once. d Comparison of changes in tumour size (rel. size) between samples from primary tumour and samples from metastases transplanted to the zebrafish larvae. The cut-off of less than 80% regression of the tumour size is shown with a red dotted line. *p < 0.05, **p < 0.01. e Comparison of number of cells disseminated to the caudal venous plexus of the zebrafish larvae between samples from primary tumours and samples from metastatic lesions. Red dotted line indicates a cut-off for more or less advanced disease (above or below respectively, see Fig. 4). **p < 0.01. Pat: patient.
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