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Mycobacterial infection-induced miR-126 expression alters the host macrophage response. (A) Measurement of whole-body neutrophil fluorescent area at 1 and 3 dpi in scramble control and miR-126 knockdown uninfected and infected embryos. (B) Measurement of neutrophil levels after trunk infection with M. marinum in miR-126 knockdown embryos. (C) Measurement of whole-body macrophage fluorescent area at 1 and 3 dpi in uninfected and infected miR-126 knockdown embryos. (D) Measurement of macrophage levels after trunk infection with M. marinum in miR-126 knockdown embryos. (E) Ratio of macrophage fluorescent area per bacterial fluorescent area at granulomas in miR-126 knockdown embryos at 1 and 3 dpi. (F) Percent of macrophages residing in the caudal haematopoietic tissue in scramble control and miR-126 knockdown embryos at 1 and 3 dpi. (G) Measurement of macrophage recruitment to a tail wound in miR-126 knockdown embryos. Data information: each data point represents a single measurement with the mean and SEM shown. For neutrophil analysis, 10–20 embryos per group were analysed and 15–50 embryos per group for macrophage analysis. For neutrophil time-lapse imaging, each data point represents the mean of six foci of infection from six separate embryos, and the graph is representative of two experimental replicates. For macrophage time-lapse imaging, each data point represents the mean of three foci of infection from three separate embryos, and the graph is representative of two experimental replicates. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
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