Fig. 1

Experimental workflow. Embryos originating from three different spawning groups were used for experiments from approx. 2 hpf onwards with 15 embryos per condition and replicate. For immunosuppression, embryos were exposed to CP dissolved in the medium from 2 hpf until the end of the experiment. At 48 hpf, embryos were transferred to new media (with CP if needed). For all conditions comprising an immunostimulation, this medium additionally contained 4000 nM IMQ. For the bioinformatical analysis of transcriptomic profiles of treated embryos, total RNA was extracted at 72 hpf and sequenced. The icons on the lower timescales indicate performed medium exchanges (droplet), microscopical inspections of apical observations (eye) and the euthanasia of embryos (skull). All conditions mentioned in the text are indicated with numbers or the respective compound. (2-column-fitting image, colour print).