Figure 6—figure supplement 1.

Salmonella early infection induces a strong activation of tumor necrosis factor (TNF)-expressing cells

(A) Tg(tnfa:GFP-F) larvae were injected with PBS (left panel) or Sal-DsRed (right panels) in the HBV. Representative maximum projections from video sequences using light sheet fluorescence microscopy starting at 1 hpi during 10 hr, showing recruitment of tnfa⁺ cells (green) to the infection site (Salmonella, red). Scale bar: 30 μm. (B) Tg(mfap4:mCherry-F/tnfa:GFP-F) larvae were injected with PBS or Sal-E2Crimson in HBV and 4D acquisition was performed using confocal microscopy from 4 to 15 hpi. Quantification of the total volume of recruited cells, mfap4⁺ or tnfa⁺ cells, from 4 to 15 hpi. Data of two replicates pooled (mean volume/larva ± SEM, n = 12 per condition, Mann–Whitney test, two-tailed, ****p < 0.0001, **p < 0.01). (C) Tg(mfap4:mCherry-F/tnfa:GFP-F) larvae were injected with PBS or Sal-E2Crimson in HBV and imaged at 4 dpi using confocal microscopy. Quantification of the total volume of recruited cells, mfap4⁺ or tnfa⁺ cells, is shown at 4 dpi. Data of four replicates pooled (mean volume/larva ± SEM, n_Sal = 23, n_PBS = 20, Mann–Whitney test, two-tailed, ****p < 0.0001).