Fig 2

Tissue culture conditions and macrophage exposure induce increased cell body size and capsule production, with differing effects on phagocytosis. (A) Cell body diameters for YPD-grown, TC-experienced, and J774-experienced (MEC) Cn. Diameters measured via widefield microscopy. Data from three biological replicates of at least 20 cells per condition per replicate. Statistical comparisons represent the results of two-tailed unpaired t-tests. (B) Capsule diameters for the same cells analyzed in panel A. Statistical comparisons represent results of two-tailed unpaired t-tests. (C) The proportion of fluorescent yeast cells inside EGFP⁺ cells (macrophages) at 2.5 hpi. Statistical comparisons represent results of one-way ANOVA with multiple comparisons. (D) EGFP⁺ cells (mpeg⁺ macrophages) phagocytosing J774-experienced Cn at ~2.5 hpi. Yeast 1 and 2 are fully engulfed, while #3 is partially engulfed. Live confocal image taken with 40×, 1.1NA water objective. 3D rendering shown. Grid lines are 20 µm apart. Animation of this data in Video S1.