Fig 5

J774-experienced Cn are more effective than YPD grown at colonizing the CNS in zebrafish but the difference is mostly accounted for by tissue culture conditions. (A) Yeast (green) outside the CNS vasculature (magenta) at 1 and 2 dpi after infection with YPD-grown Cn (left), TC-experienced Cn (middle) and J774-experienced Cn (right). Growth is subjectively greater in TC-experienced and J774-experienced Cn. Airyscan confocal images were collected with 40×, 1.1NA water objective. (B) Comparison of brain lesions per fish between historical data with YPD-grown Cn (ref), TC-experienced, and J774-experienced Cn. There is a strong trend toward fewer brain lesions per fish with YPD-grown Cn but this does not reach statistical significance with the data available. Statistical comparison for this and all other panels of this figure represents results of Mann-Whitney tests of log-transformed ratios. (C) Percent of brain lesions cleared between 1 and 3 dpi, between TC-experienced and J774-experienced Cn. The trend is toward more clearance of TC-experienced cells, but not statistically significant. (D and E) Fold change of number of yeast per brain lesion in lesions not cleared from 1 to 2 dpi (D) and 2 to 3 dpi (E).